The 24-year-old appeared to tell one Tottenham player 'I'm alright' during the session and gave an OK gesture to reassure the team-mate.
Kane was forced to sit out Tottenham's last two matches after picking up a hamstring problem in their 4-1 Premier League win over Liverpool.
Pochettino provided an update on Kane's fitness at his press conference at the Tottenham training ground.
'We're very confident,' Pochettino said when asked about Kane's availability. 'He's on the list. Maybe it's possible for him to play but I don't want to say 100 per cent'.
'He trained but it is important we make the right decision,' Pochettino added. 'With the doctors and the player, we will take the best decision for everyone. We must analyse and take the best decision for everyone.